Abstract
Deoxynivalenol (DON) is one of the most common contaminants of various foodstuffs. A biotransformation system was used in order to lessen the toxicity of DON. A strain of Aspergillus (NJA-1) was isolated from soil and cultured in an inorganic salt medium containing DON. Bt2a/Bt2b primers were used to amplify the β-tubulin gene of NJA-1. Sequence analysis the PCR product and morphology observation indicated that NJA-1 belonged to Aspergillus tubingensis (aerobic fungi). The DNA sequence information of the PCR product was deposited in GenBank (accession number DQ9025790). The DNA sequence had 99% similarity to the Aspergillus tubingensis accession number AY820009. An unknown compound in NJA-1 showed the ability to convert DON into another product. The molecular weight of the bioconversion product was 18.1 D (H2O) larger than that of DON. The analysis showed that DON could be hydrolyzed by NJA-1. The mean DON biotransformation rate was 94.4% after two weeks of cultivation. The finding presents a new method for DON biotransformation.
Highlights
Deoxynivalenol (DON), known as vomitoxin, a member of the trichothecenes which are secondary metabolites of fungi, mainly Fusarium, is one of the most common contaminants in various foodstuffs [1,2,3,4,5]
In this study the strain of Aspergillus tubingensis (NJA-1) was isolated from soil by inorganic salt culture media supplemented with DON
Sequence analysis of Bt2a-Bt2b gene and morphology observation indicated that NJA-1 belong to Aspergillus tubingensis
Summary
Deoxynivalenol (DON), known as vomitoxin, a member of the trichothecenes which are secondary metabolites of fungi, mainly Fusarium, is one of the most common contaminants in various foodstuffs [1,2,3,4,5]. The toxic effects of DON in animals have been well documented and focused mainly on the immune system and the gastrointestinal tract [7]. Many methods are used for removal of mycotoxins in moldy feedstuff including chemical, physical and microbiological treatments [13,14,15,16,17,18,19,20,21,22]. Because of DON’s relatively high chemical stability and nonpolar structure, physical treatments have a very low efficacy to remove DON. Chemical treatment has a high efficacy on DON detoxification, but it can greatly reduce nutritive value and palatability of foodstuffs. The article mainly reports the isolation and identification of NJA-1 and its efficacy on DON-transformation
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