Abstract
Phenol is widely used in China, it not only pollutes the environment, but also accumulates toxic substances in the human body through the food chain, further harming humans. In this experiment, a strain of high-efficiency low-temperature degradation phenol bacteria B5 was selected from the soil contaminated by organic matter of Lanzhou. Through research methods such as Gram staining observation, DNA extraction, PCR amplification, sequencing and comparison, it was found that this strain was Pantoea agglomerans. Through the subsequent optimization of degradation conditions, it was found that the B5 strain can degrade 500mg/L of phenol to 24.8mg/L in 36h. The ability to degrade phenol is stronger between pH5.5-pH6.0, and the ability to degrade phenol is higher in a medium containing 4-8g/L sodium chloride. This research can provide certain theoretical guidance for phenol degradation.
Highlights
Phenol is a simple phenolic organic weak acid, which is a common organic pollutant in industrial wastewater such as petroleum, fertilizer, paper making, rubber[1]
Phenol can be directly toxic to the human body through contact with the skin and mucous membranes
Resuspend the bacteria in a re-screened liquid medium containing 500 mg/L of phenol according to the wet reuse of the bacteria, and the final bacteria content reached 1g/L, 2g/L, 4g/L, 8g/L, 16g/L
Summary
Phenol is a simple phenolic organic weak acid, which is a common organic pollutant in industrial wastewater such as petroleum, fertilizer, paper making, rubber[1]. Biological treatment of phenol-containing wastewater mainly uses the metabolism of microorganisms to convert harmful phenolic pollutants into non-toxic substances [5,6], without secondary pollution, and has a large amount of treatment. For the treatment of high-concentration phenol-containing wastewater, it is necessary to dilute the phenol concentration before entering the water to reduce the toxic effect on the organism, resulting in a substantial increase in the treatment cost. If the tolerance of microorganisms to phenol can be improved and the dilution factor can be reduced, it will be of great significance to the treatment of high-concentration phenol-containing wastewater In this experiment, a microbial strain B5 that can efficiently degrade high-concentration phenol at low temperature was screened and isolated from the organic polluted soil of chemical plants, which has certain guiding significance for the treatment of high-concentration phenol-containing wastewater at low temperature in the future
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