Abstract
Aims:The aim of this study was to isolate and identify a cellulolytic bacterium from the rumen fluid of Aceh’s cattle. Biodegradation by cellulolytic rumen bacteria can be used as a source of cellulolytic bacteria that act to degrade feed fibrous material so as to improve the quality of nutrients and digestibility of feed ingredients at a cheaper price than the use of commercial cellulase enzymes.Materials and Methods:Samples were collected from rumen fluid of Aceh’s cattle in Abattoirs (RPH) of Banda Aceh city, Indonesia, isolation, and screening of cellulolytic bacteria were done in Microbiology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Indonesia.Results:The S1 isolates showed ±2.5 cm of clear zone diameter. Microscopically, this strain was found to be a Gram-negative, Bacillus. Homology and phylogenetic tree analysis of 16S rRNA showed that S1 isolate has 91% of sequence similarity with that of Enterobacter cloacae. 91% sequence homology shown in this study proved that the S1 isolate is probably either a new species or another genus of Enterobacteriaceae.Conclusion:Current study suggests that cellulose hydrolytic bacteria isolated from rumen fluid of Aceh cattle on Bushnell Haas medium-carboxymethylcellulose agar, and some potent cellulose degrading bacteria have been identified.
Highlights
Indonesia is one of the tropical countries, where ruminants are fed on lignocelluloses agricultural-products
Several colonies of cellulolytic bacteria were isolated from the rumen fluid of Aceh cattle on BHMCMC agar, and cellulolytic activity quantitatively screens using Congo red staining 0.1%
The bacterium was isolated on pH 6.0-6.5 and temperature 36-39°C
Summary
Indonesia is one of the tropical countries, where ruminants are fed on lignocelluloses agricultural-products (such as cereal straw, grass, and tree foliage). Aceh cattle are scattered in the province of Aceh and cultivated for generations as meat producers. The advantages of this Aceh cattle in addition to having a high adaptability to the bad environment are adaptation to feed which generally contains high crude fiber [1,2]. Wahyudi et al [3] have reported that the feed crude fibers were not completely converted to animal product in intensive animal farming. These materials are fermented in the rumen by microbial community including bacteria, fungi, and protozoa [4].
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