Abstract

This study was conducted to isolate the fungus Rhizoctonia solani associated with Zinnia seeds, test its pathogenicity and evaluation the effect of alcohol extract of coontail and lotus, grapefruit extract, sea bloom 29 and Bioimmune on this pathogene. This fungus was presented in 3 samples from 11 samples and was recorded for the first time on this plant in Iraq. All the isolates caused a significant reduction in seeds germination. Isolate A22 gave the highest decrease in percentage of germination (4%). This isolate gave 100% disease incidence and 96.5% disease severity compared to control (2.5%). The percentage of germination capacity and seed germination of the seed primed with PEG solution and planted in soil inoculated by this isolate was 45%. The alcoholic extract of coontail 0.2% and Bioimmune 12% caused a significant superiority in the inhibition of this isolate . Also these two extracts caused a significant reduction in disease incidence 77.5% and 72.5% and diseasse severity 72.5% and 70% compared to control 100% and 99.38%.

Highlights

  • isolate the fungus Rhizoctonia solani associated with Zinnia seeds

  • This fungus was presented in 3 samples from 11 samples

  • All the isolates caused a significant reduction in seeds germination

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Summary

Introduction

‫حسبت النسبة المئوية للإنبات‪.‬‬ ‫تأثير الفطر‪ R. solani‬في نباتات الزينيا‬ ‫أنتخبت العزلة ‪ A22‬لمفطر والتي ثبت مف خلاؿ الاختبار‬ ‫السابمؽ أنيا أشد إم ارضية مف الع لزات الأربع الأخرى وحضر‬ ‫منيا المقاح الفطري طبقا لما اتبعو ‪ Saeed‬و‪)33( Juber‬‬ ‫بعد ذلؾ وزعت تربة معقمة في أصص بقطر ‪ 15‬سـ بمقدار‬ ‫‪ 1000‬غـ تربة‪ /‬أصيص ‪ .‬أضيؼ لقاح العزلة ‪ A22‬الى‬ ‫الأصص بنسبة ‪ ،%1‬كررت المعاممة ‪ 4‬م ارت فضلاً عف‬ ‫معاممة السيطرة‪ .‬غمفت الأصص بأكياس البولي أثيميف المثقبة‬ ‫وزرعت بذور الزينيا صنف ‪ Red Zinnia‬بعد ‪ 3‬أياـ بمقدار‬ ‫‪ 10‬بذور‪ /‬أصيص ورطبت بالماء ووضعت في البيت‬ ‫الزجاجدي وفقاً لمتصميـ تاـ التعشية وفي درجة ح اررة ‪– 20‬‬ ‫‪4 27‬ـ‪ .‬بعد مضي ‪ 45‬يوماً حسبت النسبة المئوية لممرض‬

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