Isolation and genome sequencing of a novel lytic Pseudoalteromonas phage SL20.

Abstract

Phage SL20, a novel lytic Pseudoalteromonas phage, was isolated from the coastal waters of the Yellow Sea, China. The microbiological characterization demonstrated that phage SL20 was relatively stable from 35 to 55°C and the optimal pH was approximately 6.0. A latent period of approximately 24min was indicated by a one-step growth curve. The burst size was approximately 12±3 PFU/cell. The genome had a length of 120,295bp with a G+C content of 35.84%, and predicted 95 ORFs. The phylogenetic tree based on DNA helicase showed that Pseudoalteromonas phage SL20 was related to the Pseudoalteromonas phage H101 and was a member of the family Shandongvirus. The isolation and genomic analysis of SL20 has improved our understanding of host-phage interactions and the ecology of the marine bacteria Pseudoalteromonas.