Isolation and Genetic Characterization of African Swine Fever Virus from Domestic Pig Farms in South Korea, 2019.

Hyun-Joo Kim,Ji-Hyoung Ryu,Ji-Da Choi,Min-Kyung Jang,Yong-Joo Kim,Hae-Eun Kang,Ki-Hyun Cho,Jin-Ju Nah,Ha-Gyeong Chae

doi:10.3390/v12111237

Hyun-Joo Kim, Ji-Hyoung Ryu + Show 7 more

Open Access

https://doi.org/10.3390/v12111237

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Journal: Viruses	Publication Date: Oct 30, 2020
Citations: 23	License type: CC BY 4.0

Affiliation: Animal and Plant Quarantine Agency

Abstract

On 17 September 2019, the first outbreak of African swine fever in a pig farm was confirmed in South Korea. By 9 October, 14 outbreaks of ASF in domestic pigs had been diagnosed in 4 cities/counties. We isolated viruses from all infected farms and performed genetic characterization. The phylogenetic analysis showed that all of fourteen ASFV isolates in South Korea belong to genotype II and serogroup 8. Additionally, all isolates had an intergenic region (IGR) II variant with additional tandem repeat sequences (TRSs) between the I73R and I329L genes and showed characteristics of central variable region (CVR) 1 of the B602L gene and IGR 1 of MGF 505 9R/10R genes. These are identical to the genetic characteristics of some European isolates and Chinese isolates.

Highlights

African swine fever (ASF) is a fatal viral disease that affects domestic and wild pigs of all ages around the world
Since ASF was introduced to Georgia from Africa in 2007, it has spread to neighboring countries including Russia, and EU member countries in 2014
On 3 October 2019, the first carcass of a wild boar infected by ASF virus (ASFV) was found in the demilitarized zone (DMZ), at a distance of 8.4 km from the 14th ASF outbreak pig farm in Yeoncheon county [3]

Summary

Introduction

African swine fever (ASF) is a fatal viral disease that affects domestic and wild pigs of all ages around the world. The first ASF case in South Korea was reported on 16 September 2019 at a pig farm located in. We reported that the first ASFV isolate Korea/Pig/Paju1/2019 belongs to genotype II and has an additional tandem repeat sequence in the intergenic region (IGR) between the. As a result of these studies, the classification of the serogroup-based EP402R gene, different types of central variable region (CVR) of the B602L gene, and kinds of tandem repeat sequence (TRS) insertion in the IGR of multigene family (MGF) 505 9R/10R genes were reported [4,5,6,7]. We isolated ASFV from 14 infected domestic pig farms and identified both the characterization of isolates in vitro and additional genetic properties in this study

Virus Isolation

PCR Assay

Analysis of the ASFV Isolates

Results and Discussion