Abstract
Today’s chrysanthemums are highly evolved flowering plants and they are considered as one of the most important ornamental cut flowers. In this research an isopentenyl transferase gene named CmIPT1 was isolated from Chrysanthemum morifolium cv. ‘Jinba’ using RACE and RT-PCR methods. The full cDNA sequence of CmIPT1 was 873 bp which encoded a deduced protein of 290 amino acids. It contained GxxGxGKS which is a conserved sequence of the typical domain of IPT family. The phylogenetic tree analysis of CmIPT1 in Chrysanthemum morifolium cv. ‘Jinba’ shows that it has the closest relationship with CcIPT1 in Cynara cardunculus var. scolymus. Expression of CmIPT1 was higher in stems and apex, whereas it was lower in leaves and roots. And the overexpression of CmIPT1 obviously increased the number of rosette branches in Arabidopsis. Here, in our study, we showed that CmIPT1 is a positive regulator of branch development in Chrysanthemum and may play a key role in regulating lateral branch formation of Chrysanthemum plants.
Highlights
The development of branching in plants involves the formation and subsequent growth of axillary buds [1]
In this research an isopentenyl transferase gene named CmIPT1 was isolated from Chrysanthemum morifolium cv
Based on the available IPT1 sequences from plant species such as Brassica campestris, Arabidopsis thaliana, Raphanus sativus and Solanum tuberosum, a 781 bp length DNA fragment was obtained from Chrysanthemum morifolium using PT1-PF/IPT1-PR
Summary
The development of branching in plants involves the formation and subsequent growth of axillary buds [1]. Research showed that the level of expression of PsIPT1 and PsIPT2 in peas increased significantly and axillary bud started germinating after removing the shoot top by pruning. This illustrated that cytokinin was synthesized promptly to accelerate the growth of axillary buds after removing the shoot top by pruning [15]. According to the research results, IPT1 plays an important role in regulating lateral branch formation and the development of plants. We studied the role of the CmIPT1 gene in regulating branching by using allogenic transformation of Arabidopsis with the aim of breeding new varieties of cut-flower Chrysanthemum having less lateral branches or without any lateral branches by means of molecular biology techniques
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