Abstract
BackgroundThere have been four strains on Manzanilla virus (MANV) identified to date. Here, we identify a novel MANV strain (DHL10M107) isolated from Culex tritaeniorhynchus Giles mosquitoes from Ruili city, Dehong prefecture, Yunnan Province, in the People’s Republic of China.ResultsThe DHL10M107 L, M and S genes were sequenced at the nucleotide and deduced amino acid levels. The L, M and S gene sequences of DHL10M107 clustered with the MANV strains VN04-2108, TRVL3587, SA An 4165, and AV 782. DHL10M107 was most closely related to VN04-2108. Nucleotide homology ranged between 96 and 99% between DHL10M107 and VN04-2108. In terms of amino acid homology, all of the amino acid differences were in the L (96.3% homologous) and M (97.7% homologous) fragments.ConclusionsDHL10M107 is likely a MANV isolated from mosquitos in the Yunnan Province. This is the first reported isolation of MANV in mainland China.Electronic supplementary materialThe online version of this article (doi:10.1186/s13104-015-1198-5) contains supplementary material, which is available to authorized users.
Highlights
There have been four strains on Manzanilla virus (MANV) identified to date
Phylogenetic analysis of the viral L and M gene sequences indicated that DHL10M107 clustered with MANV (VN04-2108) and an Oya virus (OYV) strain (SC0806) isolated from mosquitos in Sichuan province, China [17], it was more closely related to VN042108 (Figure 1a, b)
Regardless of the genome fragment, DHL10M107 was closely related to three MANV strains
Summary
There have been four strains on Manzanilla virus (MANV) identified to date. Here, we identify a novel MANV strain (DHL10M107) isolated from Culex tritaeniorhynchus Giles mosquitoes from Ruili city, Dehong prefecture, Yunnan Province, in the People’s Republic of China. MANV is a single-stranded negative sense RNA virus that contains three RNA fragments: large (L), medium (M) and small (S). In 2004 Bryant et al isolated a MANV strain identified as the Cat Que virus (VN04-2108) from mosquitoes (Culex sp.) in Vietnam [2]. In 2014, Ladner et al [3] reclassified two viruses as MANV strains, the Ingwavuma virus
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