Abstract
The high-molecular-weight polynuclear aromatic hydrocarbon (PAC) fraction of a carbon black extract was isolated with a backflush technique, and applied to an amino-bonded stationary phase for HPLC. This fraction was further separated into 44 sub-fractions by means of reversed-phase HPLC, on an ODS column. Low-temperature fluorescence, utilizing the Shpol'skii effect, was then applied to each fraction. By this method, quasilinear spectra were recorded from mostly peri- condensed PACs with molecular weights up to 400 daltons. Nine compounds with molecular weights exceeding 300 daltons were identified.
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