Year-end Sale % OFF 
Abstract
Native endophytic actinomycetes isolated from pearl millet roots were examined for their efficacy to protect pearl millet against downy mildew. Nineteen of 39 isolates were found to be proteolytic, of which 7 strains could directly suppress the sporangium formation of Sclerospora graminicola, the pearl millet downy mildew pathogen. Thus, mycelial suspensions containing either spores or cell-free extract of these 7 isolates were used for seed-coating and -soaking treatments to test for their induction of downy mildew resistance. Results indicated that seed-coating overall provided better protection to downy mildew than seed-soaking. In both treatments, the tested isolates demonstrated differential abilities in downy mildew disease protection, with Streptomyces griseus SJ_UOM-07-09 and Streptosporangium roseum SJ_UOM-18-09 showing the highest protection rates. Additionally, the levels of disease protection conferred by the actinomycetes were just slightly lower than that of the systemic fungicide Apron, suggesting their effectiveness. Further studies revealed that the more rapid root colonization by SJ_UOM-18-09 resulted in faster and higher induced resistance in comparison with SJ_UOM-07-09 under greenhouse conditions, indicating that SJ_UOM-18-09 was superior than SJ_UOM-07-09 in inducing resistance. Results from this study provide comprehensive information on biocontrol functions of SJ_UOM- 18-09 with great potential to control downy mildew disease in pearl millet.
Highlights
Downy mildew disease caused by Sclerospora graminicola is responsible for worldwide yield losses on pearl millet (Pennisetum glaucum)[12]
Classification of identified endophytic actinomycete strains isolated from pearl millet roots using 16S rRNA sequencing and morphological assays
A total of 47 isolates were obtained from roots of the pearl millet cv. 1P18192 that has been known to be resistant to downy mildew disease[18]
Summary
Downy mildew disease caused by Sclerospora graminicola is responsible for worldwide yield losses on pearl millet (Pennisetum glaucum)[12]. Various types of induced resistance have been reported to control crop diseases[14,16,17]. It is important that the bio-agents from native soils with an array of beneficial traits[17,19,20,21] on crop improvement should be exploited. In this context, the present work was undertaken to isolate and test the efficacy of proteolytic actinomycete isolates in inducing protection against downy mildew of pearl millet. The isolated actinomycetes were characterized for their beneficial characteristics that enable them to confer protection against downy mildew caused by S. graminicola
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
