Abstract
Clerodendrin A, a chemical marker was isolated from the root of Clerodendrum phlomidis (Verbanaceae). We here report first time, the presence of clerodendrinAin another Verbanaceae drug, Premna integrifolia. Further, HPTLC method was developed to quantify isolated clerodendrinAin C. phlomidis and P. integrifolia roots using n-hexane: ethyl formate (7:3) as mobile phase, pre-coated TLC plates (silica gel 60 F 254 ) as stationary phase and H 2 SO 4 as derivatizing agent. The method was validated in terms of linearity, precision, repeatability and accuracy. The limit of detection and limit of quantification was determined on the basis of signal to noise ratio. Content of clerodendrin A was found to be 0.073 and 0.04% w/w in C. phlomidis and P. integrifolia respectively. The developed HPTLC method was easy, simple, and precise.
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