Abstract
Chondrocytes from superficial layers of articular cartilage have distinct phenotypic properties which are different from those of cells obtained from the deeper areas. We describe a method that isolates highly purified articular cartilage chondrocytes from the superficial layers. When the superficial cells are stimulated in vitro with a source of cytokines, they secrete greater amounts of metalloproteinase compared to chondrocytes obtained from a deeper area.
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