Isolation and Diagnosis of Most Frequent Respiratory bacterial Infection from aged cases with and without Kidney Failure in Al-Diwaniya City

Abstract

Several types of bacteria (most frequent) were isolated and its responsible on respiratory system infection , 150 sputum specimens were collected from older patients (infected and non-infected kidney failure) coming to Al-Diwaniya Teaching Hospital; they were distributed among 75 samples from patients with respiratory system infection (group control) and 75 other samples from patients with respiratory system infection and kidney failure. Under study bacterial species were diagnosed by conducting a series of biochemical tests on it, as well as to confirm the diagnosis by Vitek compact 2 system. Results of isolation showed that two types appear of single and mixed infections, which made both of higher isolation by S. pneumoniae by rate of 16.00% and 13.33% in the two groups of control and kidney failure, respectively and followed by S. pyogens in second place by (12.00 and 9.33)%, then S. viridans in third place and recorded equal proportion isolate (9.33%) for both groups as well as it recorded a mixed infections - bilateral (S. pneumoniae + S. pyogens) the highest percentage isolated in the control group (22.67%) compared to kidney failure group (20.00%), while the mixed infections - tripartite had all isolates were higher in kidney failure group than in the control group. Pharmacological sensitivity tested to under study bacterial isolates to ten antibiotics had commonly used to find out how to resist it; S. pneumoniae had shown full and intermediate resistance to the majority of antibiotics under study except Penicillin G, Ciprofloxacin, Amikacin and Tetracycline that had shown sensitivity reached (55.22, 82.55, 80.00 and 60.00)%, respectively, and the same thing applies to S. pyogens except to Tetracycline resistance by 60.00% and equal to resistance and sensitivity (50%) of Cephalothin. S. viridans was sensitive to Penicillin G, Ceftazidime, Ciprofloxacin and Amikacin by (81.55, 81.10, 83.67 and 66.34)%, respectively, while it was given equal resistance and sensitivity (50%) to Ampicillin and Cephalothin, and resisted to other antibiotics. Staph. aureus givensensitivity only to Ciprofloxacin and Amikacin by (88.00 and 54.50)%, respectively, compared to their resistance to other antibiotics. Haemophillus influnza showed that complete resistance to Cephalothin, Tobramycin and Tetracycline, and given semi-complete resistant for Penicillin G and Piperacillin by (85.10 and 75.30) % respectively, while the sensitivity and resistance by Haemophillus influnza were equal to Ciprofloxacin and Amikacin along with intermediate sensitivity to Cefotaxime and Ceftazidime by (55.00 and 59.79)%, respectively. Ps. aeruginosa were fully resistant to under study antibiotics majority except for intermediate sensitivity to Cefotaxime and Ceftazidime by (60.00 and 64.36)%, respectively, along with equal resistance and sensitivity to Ciprofloxacin and Amikacin.