Abstract
In this study, 63 oral swabs were collected . Thirty six yeast isolates were obtained (57.14%) grown on sabouraud dextrose agar medium (SDA). Symbols were given to isolates from RA1 to RA36 in order to differentiate them. The isolates were identified by growth on HiCrome Candida differential agar M1297A and identification by Vitek 2 Compact system. The identification results showed 5 species of the isolated yeasts belonging to the genus Candida, 29 isolates belonging to the species C. albicans (80.55%) and one species from C. famata, C. glabrata, C. kefyr and C. lusitaniae (2.77% for each species). Moreover one isolate was obtained from each of the following yeasts: Saccharomyces serevisiae, Malassezia furfur and Rhodotorula glutinis (2.77% for each one). This study included the evaluation of two virulence factors for all C. albicans isolates , phospholipase enzyme production on egg yolk agar and hemolytic activity on sugar-enriched sheep blood agar. . Phospholipase production test showed differences in phospholipase production among the isolates , twenty three isolates (79.31%) were producers and precipitation zone (Opaque zone) was observed around the grown colonies with different Pz values and the isolate RA13 exhibited highest activity (Pz value = 0.53). The results showed also that all isolates were producers of hemolysin with different hemolytic indexes (Hi). Isolate RA7 represented the highest one in activity (Hi = 2.16). Hemolysis of all isolates involved beta hemolysis.
Highlights
This study included the evaluation of two virulence factors for all C. albicans isolates, phospholipase enzyme production on egg yolk agar and hemolytic activity on sugar-enriched sheep blood agar
The results showed that all isolates were producers of hemolysin with different hemolytic indexes (Hi)
)34( Species- specific hemolysins ان المعلومات المتوفرة عن عامل الض اروة الهممولایسین ما ازلت قلیلة ،وهناك حاجة الى المزید من الد ارسات من اجل الكشــه عن طبيعة هذا العامل المحلل للدم Hemolytic factorفي الـــــــــ C. albicansوعن فائدته في التشــخيص والكشــه بشـــــحل رئيس عن تا یره على خلایا العائل ،وقد یشـــــبه هذا العامل الانزیمات خارج خلویة ،وبذلك یســـــاعد في تمییز الع لزات ( .)13كما أن الد ارسـات حول انتاج الهيمولایسـین من قبل ع لزات الــــــ ـ Candidaوالمعزولة من الفم ما ازلت محدودة ،وإن أول د ارســـة أجریت حول فعالية تحلیل الدم لع لزات من الــــــــــ C. albicansوالمعزولة من التجوی الفمو كانت من قبل Tsang وآخرین ( )30الذین لاحظوا أن الــــــــ ـ C. albicansالمعزولة من فم المرضــى المصــابین بمرض الســحر ذات إنتاجية أعلى لـــلـــهـــيـــمـــولایســــــــــیـــن بــــالـــمـــقــــارنــــة مـــع عـــ لزات الســــــــــيـــطـــرة والـــتـــي مصــــــــــدرهــــا أةــــــــــخــــال أصــــــــــحــــاء ( )34وربمــــا قد یحون لزیــادة تـــــركیز الكــــلوكوز في اللعــاب تـــأ یر على إنتاجیــة الهيمولایســـین في عـــ لزات الـ C. albicans ( ، )30وهذا ما أكده Mannsوآخرون ( )21في د ارسـتهم التي لاحظوا فیها عدم وجود فعالية إنتاج الهيمولایسـین في ع لزات الـ
Summary
This study included the evaluation of two virulence factors for all C. albicans isolates , phospholipase enzyme production on egg yolk agar and hemolytic activity on sugar-enriched sheep blood agar.
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