Isolation and Diagnosis of Acinetobacter Bumanniii Recently Isolated From Patients in Kirkuk Hospitals and Study their Antibiotics Resistance

Abstract

Two hundred samples of clinical samples were collected from different sources from Kirkuk General Hospital, Azadi Teaching Hospital, Children Hospital and the Specialized Center of Respiratory and Chest Diseases clinics in Kirkuk city from the period Feb., 15, 2018 to Jun., 15, 2018 from out- and in-patients after consultation with the specialist doctor and the samples were forwarded to the laboratory. The data of each of the samples were recorded in special forms and they included: 55 samples of respiratory tract infection, 25 blood samples, 30 samples of meningitis, 30 samples of urinary tract infection, 30 wound samples and 30 burn samples. The isolates were identified after being cultured on MacConkey agar, and Blood agar. The biochemical tests: Oxidase and Catalase were done for the samples and for final diagnosis API 20E system was used. After culture and diagnosis, 12 isolates of Acinetobacter baumannii were obtained, of which five were from sputum, three from swabs of burns, two swabs of wound and an isolate from each of bacteremia and CSF. The results of the sensitivity tests for A. baumannii isolates from different clinical sources from Kirkuk hospitals for 13 different antibiotics showed resistance of the bacterial isolates of the study at different rates. All the samples (100%) showed resistance to Ampicillin, Amoxicillin, Ceftazidim, Cefotaxime, Chloramphenicol and Trimethoprime, while 83.33% showed resistance to Amikacin, Lefofloxacin, Trimethoprime-Sulphamethoxazole and Piperacillin, and 58.33% to Amoxicillin-Clavulanic acid and Gentamycin and 50 % to Imipenem . (50)%.