Isolation and Diagnoses of Some Microbial and Chemical Pollutants in Raw Milk Samples Which Collected From Some Regions in Salah al-din Province

Abstract

Samples of raw milk were collected from different regions in salah al-din province which marketing to one of ice houses and appropriation to the collection and cooling of raw milk storage at (5 Co) for 24-48 hours before rew marketing to the dairy plants or dealers, the number of samples which taken were (20) Samples. Swabs were taken from the udder of cows belonging to suppliers to determine the causes and incidence of microbial contamination. It was after that diagnosis types of micoorganisms that can found in such circumstances of storage and study their properties with focus on Listeria monocytogenes.
 It was farther determine the degree of contamination of milk samples with some heavy metals, Which including, cadmium, Lead , Zinc , Copper.
 Many types of bacteria were isolated and dignosed belongs to aerobic bacteria and coli from group of lactose fermented species, E. Coli and Klebsiella sp. And non lactose fermented which is Enterobacter aerogenosa, L. monocytogenes and staphylococcus aureus bacteria isolated, as well as yeast and molds , and the rate of total number reached: 5.78, 5.95, 5.71, 2.53, 2.33 log10 cfu/ml. Isolation function are also given other types of bacteria which belongs to Bacillus sp. , Pseudomonus sp., Staph. Aureus , and Micrococcus sp. In 8 swab samples.
 The results showed that the highest concentration of heavy metals of Cd, Pb, Zn, Cu in raw milk samples was 0.18, 0.25, 2.10, 1.05 mg/lit respectively. A sensitivity test of L. monocytogenes toward a number of antibiotics was found that this bacteria sensitive towards both of Ampicillin, Chloramphinicol, Erythromycin, and Tobbramycin. But it is resistant to antibiotics, Naldixic acid, Novabiocin , and cefotaxime.