Isolation and determination of antibiotic resistance patterns of urinary tract infections pathogens in patients who referred to Pol-e Dokhtar medical centers during 2016-2018

Abstract

Background: Urinary tract infection (UTI) is one of the most common human bacterial infections. The frequent use of antibiotics is associated with increased resistance of many bacterial agents to the treatment.Materials and Methods: A total of 9456 urine specimens were collected from patients referring to Pol-e Dokhtar health centers. All urine samples were inoculated on blood agar plates (BAP) and eosin methylene blue (EMB) agar. Those specimens positive for selected pathogen were finally identified. The antimicrobial susceptibility and resistance patterns of the UTI pathogens to common antibiotics were determined using disk diffusion method according to Clinical and Laboratory Standards Institute (CLSI) guidelines.Results: Out of 9456 investigated urine samples, 1036 samples (10.95%) were positive, of which 823 samples (79.4%) belonged to female subjects and 213 samples (20.6%) belonged to males. The most common identified urine tract pathogens were Escherichia coli (69.4%), Staphylococcus epidermidis (15.15%) and Staphylococcus aureus (7.05%). Also, co-trimoxazole (sulfamethoxazole/trimethoprim) displayed the highest antibiotic resistance (58.1%) and nitrofurantoin (90.1%) and gentamicin (88%) were shown to be the most frequent cause of sensitivity.Conclusion: The findings of our study demonstrate that E. coli is the most common UTI pathogen. Given the high antibiotic resistance of co-trimoxazole, it is recommended to avoid indiscriminate consumption of this antibiotic and perform antibiogram tests for the treatment of UTIs.