Abstract
Objective: This research was conducted to isolate the alkaloid carpaine by chromatography method and to determine it quantitatively by Thin Layer Chromatography Scanner.
 Methods: Dried leaves were macerated with ethanol 70% and fractionated with dichloromethane. Isolation of carpaine alkaloid from the dichloromethane fraction was carried out by column chromatography and preparative thin-layer chromatography according to the Rf value in Thin Layer Chromatography (TLC) after exposure by Dragendorff reagent.
 Results: The content of carpaine alkaloid was 7.5 mg with Rf 0.58 and dichloromethane: methanol (9.2:0.8) as eluent. Validation showed the linearity (R2) 0.9988, the limit of detection(LOD) was 0.05 ppm, the Limit of Quantification (LOQ) was 0.19 ppm, the recovery from 98.93-102.43%, and the % coefficient of variation was 0.16%.
 Conclusion: Carpaine alkaloid in papaya leaf extract was 10.52%.
Highlights
Papaya (Carica papaya L.) comes from the family Caricaceae
Each sub-fractions were monitored by Thin Layer Chromatography (TLC) using dichloromethane: methanol (9:1) as mobile phase; the eluted plates were exposed to Dragendorff reagent
Each sample solution was placed in the TLC plate, measured by a TLC scanner. This plant was taxonomically determined at Plants Taxonomy Laboratorium, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran as Carica papaya L. of the family Caricaceae, collection-number 476/HB/01/2017
Summary
Papaya (Carica papaya L.) comes from the family Caricaceae. This plant is cultivated in almost all subtropical and tropical countries such as South America, India, Phillippines and Indonesia. Papaya leaves can significantly increase platelet counts and increase the number of red blood cells [11] and showed analgesic activity in mice compared to aspirin [12]. The major component of papaya leaves is carpaine alkaloid. This compound reported can reduce blood pressure, antitumor and antiplasmodial activity [14]. The isolated carpaine is able to maintain mouse platelets up to 555±85.17 x 109/L which has been induced by busulfan for 20 d with a dose of 200 mg/kg papaya leaf extract containing 2 mg/kg carpaine alkaloid [16]
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