Isolation and Detection of UreC and UreR of Proteus mirabilis in Gastrointestinal Samples from Patients with Crohn's Disease in Iraq

Abstract

Proteus mirabilis is a Gram-negative facultative anaerobe bacillus isolated from the human gastrointestinal tract that has recently been linked to Crohn's disease (CD) recurrence after bowel resection. CD is an inflammatory bowel disease that affects the entire gastrointestinal tract, including the mouth and anus. Inflammation, ulcers, and other damage to the digestive tract lining characterize it. In the current study, CD patients as well as controls provided 79 biopsy samples of both sexes and ages from the Teaching Hospital Gastroenterology and Hepatology, medical city, Baghdad, from October 2022 to March 2023, to detect Proteus mirabilis P. mirablis bacteria, as well as the results of biochemical tests and the Vitek 2 system. P. mirablis was subjected to a PCR assay using particular primers that targeted the genes ureR and Urease C (ureC), which encode for the urease enzyme, a virulence factor in Proteus species.