Abstract
A total of 50 fecal samples were collected from buffalo calves ages between 3 days to 4 months, in middle area of Iraq (Baghdad, Salahaldden, Babylon, Diyala, and Wasit) between July 2016 to June 2017. All samples were examined by Immuno-chromatographic rapid tests for detection of buffalo rotavirus, twenty samples were positive. Enzyme-Linked Immunosorbent Assay. test was also used and revealed only 8 positive samples, the later samples were used for viral isolation on fetal bovine kidney cell culture for detection the cytopathogenic effects of the virus. The cytopathogenic effects of virus were induced rounded cells and gaps; as a result of rupture of these infected cells by rotavirus. Moreover Enzyme-Linked Immunosorbent Assay test used again for detection of rotavirus cytopathogenic effect in fetal bovine kidney cell culture for more confirmation. The TCID 50 was estimated for rotavirus on fetal bovine kidney cells 104.5 virus/0.05 ml
Highlights
1: Results of ELISA test for detection rotavirus in 20 fecal samples according to age of tested calves revealed that 50% positive
Immunoassay according to age of calves in the five governorates (10 for each one)
In the current study all the 20 positive samples from chromatographic immunoassay were tested by ELISA method to reveal the cell culture causing cytopathogenic effect (CPE) after 3rd passage of RV, the cells appeared rounded at second day post infection, large syncytia formation after
Summary
A total of 50 fecal samples were collected from buffalo calves ages between 3 days to 4 months, in middle area of Iraq (Baghdad, Salahaldden, Babylon, Diyala, and Wasit) between July 2016 to. Test was used and revealed only 8 positive samples, the later samples were used for viral isolation on fetal bovine kidney cell culture for detection the cytopathogenic effects of the virus. Enzyme-Linked Immunosorbent Assay test used again for detection of rotavirus cytopathogenic effect in fetal bovine kidney cell culture for more confirmation. 1: Results of ELISA test for detection rotavirus in 20 fecal samples according to age of tested calves revealed that 50% positive
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