Isolation and detection of microRNA from the egg of chickens.

Ben Wade,Tamsyn M Crowley,Anthony Keyburn,Michelle Cummins

doi:10.1186/s13104-016-2084-5

Abstract

BackgroundThe egg is a vital part of the chicken developmental process and an important protein source for humans. Despite the chicken egg being a subject of intense research little attention has been given to the role of microRNAs within the egg.FindingsWe report a method for the reproducible and reliable isolation of miRNA from the albumen and yolk of chicken eggs. We also report the detection via real-time PCR of a number of miRNAs from both of these biological fluids.ConclusionsThese findings provide an interesting look into the chicken egg and raise questions as to the role that miRNAs maybe playing in the chicken egg. This method of detecting miRNAs in chicken eggs will allow researchers to investigate the presence of an additional level of epigenetic programming in chick development previously unknown and also how this impacts the nutritional value of eggs for human consumption.

Highlights

The egg is a vital part of the chicken developmental process and an important protein source for humans
These findings provide an interesting look into the chicken egg and raise questions as to the role that miRNAs maybe playing in the chicken egg
This method of detecting miRNAs in chicken eggs will allow researchers to investigate the presence of an additional level of epigenetic programming in chick development previously unknown and how this impacts the nutritional value of eggs for human consumption

Summary

Conclusions

These findings provide an interesting look into the chicken egg and raise questions as to the role that miRNAs maybe playing in the chicken egg. These findings provide an interesting look into the chicken egg and raise questions as to the role that miRNAs maybe playing in the chicken egg This method of detecting miRNAs in chicken eggs will allow researchers to investigate the presence of an additional level of epigenetic programming in chick development previously unknown and how this impacts the nutritional value of eggs for human consumption

Background

Methods

Results and discussions