Abstract
Listeria monocytogenes (L. monocytogenes) is the food borne pathogen responsible for listeriosis, which is considered a serious public health risk and are the most important pathogens which can be spread through food products consumption. The disease that can be serious and fatal to human and animals. The objective of present study was carried out to detect, isolate and identify L-monocytogenes from frozen chicken, minced meat and cheese in duhok province. Within a period of six months from march to October 2015, a total of 150 samples were collected including 50 samples of minced meet, 50 samples of frozen chickens and 50 samples of cheese. Biochemical and microbiological test with Real time PCR technique by using specific primer was performed to determine the prevalence of the L-monocytogenes in the samples. Detection of such bacteria in different kinds of food product is crucial to safeguard public health due to it is potential hazard in human and animals. Out of 150 samples 20 samples displayed garish and black colonies with black halos on Oxford and Palcam agar. From the total of 20 suspected Listeria 12 isolate of L. monocytogenes identified by PCR. L. Monocytogenes were detected in 1(2%), 7(14%), and 4(8%) isolates from cheese, minced meat and frozen chicken respectively. The PCR technique has demonstrated to be a rapid and reliable method appropriate for the routine analysis of different types of food.
Highlights
Listeria spp. is broadly distributed in environment
The findings of these results indicated that the meat products were considerably more likely to be contaminated with L. monocytogenes than other food products
It should be noted that the pathogenic L. monocytogenes in our research for the contamination rate of Listeria in dairy products, cheese are consistent with the results indicated by Jalali and his team [16]
Summary
Listeria spp. is broadly distributed in environment. It can be isolated from soil and water because the organisms of the Listeria are omnipresent in nature [1]. A wide range of animal species can be contaminated by L. monocytogenes, including mammals, domesticated animals, pets, fish, birds and crustaceans [2]. Almost all cases of listeriosis tend to be foodborn, and a number of food items could be contaminated by L. monocytogenes including raw chicken meat, raw minced meat, soft cheese and fish. Most of these items are widely consumed in Kurdistan and Duhok province, especially [6]
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