Abstract
Protoplasts were isolated enzymatically from friable callus cultures of Ulmus x ‘Homestead’. Protoplasts plated in a liquid KM medium containing 0.38 M glucose, 0.7 mM sucrose, 1.0 μM 2,4-dichlorophenoxyacetic acid (2,4-D), 1.0 μM naphthaleneacetic acid, and 0.5 μM zeatin underwent cell division as early as 11 days and formed multicellular colonies by 20 days after isolation. Plating efficiency was dependent upon the initial plating density with the optimal plating density being 0.5 × 10 5 protoplasts per ml.
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