Abstract
Protoplasts were enzymatically isolated from callus of Gossypium klotzschianum Anderss. with a 20.5% yield. Isolation efficiency was influenced by age of callus, incubation time, concentration of osmoticum and agitation speed. The enzyme incubation mixture consisted of 1% Cellulysin, 0.5% Macerase and 0.7 M mannitol at pH 4.7. Divisions of protoplasts were first observed 3 days after isolation and multicellular colonies were formed by 2 weeks.
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