Isolation and characterization of Ureaplasma parvum from the semen of infertile men and detection of virulence factors genes

Abstract

The bacterial infection of seminal fluid is one of the most important factors behind infertility in many cases. The biological diversity of the Ureaplasma and Mycoplasma have widely been reported to be associated with the infertility grade in many cases. The purpose of this research was to identify and characterize and the phylogenetic positioning of twenty-one isolates of Ureaplasma in seminal fluids. Two genetic loci were included in this study ureB/ureA and upV for Ureaplasma. Each amplified locus was investigated in both bacterial infections to identify the identity of the possible bacterial infection of those patients and to assess the pattern of the genetic variation for each genetic locus in investigated patients. The results of the DNA sequence analysis of the urease and upv gene extracted from these bacteria showed that Ureaplasma parvum serovar 3 was the cause of infections in the studied samples. The studied isolates were also matched with the global isolates registered in the gene bank. After NCBI matching for these gene segments, polymerase chain experiments confirmed the diagnosis. The NCBI BLASTn The algorithm discovered up to 99.5 percent sequence similarity. between the studied samples and the reference samples for these gene.