ISOLATION AND CHARACTERIZATION OF UNIQUE FRUCTOPHILIC LACTIC ACID BACTERIA  FROM DIFFERENT FLOWER SOURCES.

G. M. Saleh

doi:10.36103/ijas.v51i2.977

Abstract

This study was aimed to investigate Lactic acid bacteria (LAB) that contain an important group of bacterial strains such as fructophilic lactic acid bacteria that usually isolated from fructose rich niches. These groups of bacteria have been considered as probiotics, especially in animal applications. In this study these bacteria were isolated from 6 Plant sources (flowers) such as: Convolvulus arvensis, Hibiscus rosa-sinensis , Nerium oleander, Rosa rugosa, Tagetes erecta and Zinnia elegans. The bacterial isolates were identified by morphological and molecular methods through the detection of 16SrRNA gene. Carbohydrate fermentation profile as well as their antibacterial activity using the dual culture overlay assay was also detected. The results revealed that 5 types of different lactic acid bacteria were isolated included: Fructobacillus fructosus, Lactobacillus kunkeei, Enterococcus durans, Enterococcus faecium , and Lactobacillus brevis. These isolates showed variety in fermentation of carbohydrates. Also, all isolates exhibit inhibition effect against pathogenic bacteria (Staphylococcus auresو Pseudomonas aeruginosa) except Enterococcus faecium which showed no effect it.

Highlights

‫تحتوي بكتيريا حامض اللكتيك على مجموعة مهمة من الانواع البكتيرية والتي تعرف ببكتيريا حامض اللكتيك المحبة‬
The results revealed that 5 types of different lactic acid bacteria were isolated included: Fructobacillus fructosus, Lactobacillus kunkeei, Enterococcus durans, Enterococcus faecium, and Lactobacillus brevis
Convolvulus arvensis and Nerium oleander gave Staphylococcus epidermidis and Enterobateriaceae with no presence of Lactic acid bacteria (LAB), whearase Tagetes erecta gave L.brevis isolate; Hibiscus rosa-sinensis gave L.kunkeei, E.durans and E.feacium isolates ;Rosa rugoso gave L.kunkeei, L.brevis and F.fructococus isolates, while Zinnia elegans have the most diverse bacterial component

Summary

INTRODUCTION

Plant like leaves, stalk, fruits and roots [25,26]. Fructophilic lactic acid bacteria (FLAB) is a Previous studies mentioned that flowers were special group of Lactic acid bacteria (LAB) considered unsuitable for bacterial growth with unique characters. The idea of transmission of these when the receptor is absent they ferment D- FLABs between flowers and honeybees that glucose poorly [14] This unique character visit them has raised an important question, differentiated them from their main group of whether the transmission is from the flowers to LAB. A hypothesis cocci, non-spore formers and generally non- suggested that the FLAB can affect the motile They habitat environments rich in chemical composition of floral nectar, and as a sugars, vitamins, amino acids, minerals and result attracting the honeybees toward the derivatives of nucleic acids [21]. The FLAB for honeybees health and humans as well This group was further divided into obligate and study was aimed to shed light on the facultative fructophil bacteria in which both predominant FLAB and LAB species isolated. The flower part of a rugosa, Tagetes erecta and Zinnia elegans, plant determines the type and availability of their blooming time, size and colors are shown nutrients which differ from other parts of the in, (Table 1), (Fig .1)

Saleh e f d d

PCR conditions consisted of an initial

RESULTS AND DISCUSSION

Phylogenic identification

Expect Identities Position in Gene Bank

Lactobacillus kunkeei

Antibacterial activity test of bacteriocin from