Abstract
The Suppression Subtractive Hybridization (SSH) method was applied to construct a cDNA library from Gossypium barbadense cv. ‘Pima 90’. Two dirigent-like (DIR) genes, Gbd 1 and Gbd 2, were isolated from the library. RT-PCR and Northern blot showed that they were induced after infection by Verticillium dahliae. The nucleotide sequences of Gbd 1 and Gbd 2 were 705 bp and 801 bp long with open reading frames of 531 bp and 525 bp, respectively. Sharing high homologies to disease resistance-responsive family proteins of Arabidopsis thaliana, Gbd 1 and Gbd 2 shared a DIR-conserved domain and possessed a hydrophobic N-terminal signal peptide. Gbd 1 and Gbd 2 encode a class of cellsurface proteins with signals for receptor-mediated plant defense/immunity activities. The possible function of the genes and the mechanism of disease-resistance of ‘Pima 90’ are discussed.
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