Abstract

Twenty-one microsatellite loci were developed for the tropical ascidian, Polycarpa aurata. Microsatellite enriched genomic libraries were constructed and sequenced using Pacific Biosciences single molecule real time sequencing technology. The 21 loci were characterized in 20 individuals from Alor, Indonesia. Observed levels of heterozygosity ranged from 0.30 to 1.00 with a mean of 8.2 alleles per locus. Loci PA21 and PA45 showed evidence of significant linkage disequilibrium and three loci significantly deviated from Hardy–Weinberg expectations.

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