Isolation and Characterization of the TATA binding protein (TBP) of Taenia solium

Abstract

Taeniosis/cysticercosis are diseases caused by Taenia solium and mainly affects developing countries. Little it is known about the transcriptional system in cestodes, therefore we want study and found the differences with the human and pig (host) transcription system.The aim of this study was the identification of the elements present in the core promoter that regulates the formation of the pre initiation complex (PIC). We analyze the core promoter of different genes and identify (in silico) the cis‐elements presents. Moreover, we have cloned and characterized a TBP in T. solium. TBP is an important transcription factor to form the PIC. Besides, we identified the gene that encodes this TBP. The gen shows the presence of 4 introns in accordance with Chambon's rules and the recognition sites for splicing machinery. The amino acid sequence translated from cDNA reveal a 238‐residues protein with an expected weight of 26.7 kDa. Southern and northern blot analysis suggests the presence of one gene and two mRNA for TBP in T. solium. EMSA and supershift show a specific interaction between the TATA box of different promoters and nuclear extracts from T. solium. Our results indicate that we have isolated the gen encode a TBP and showed it interaction with TATA box from different genes.This work was supported by grant IN219711 and CONACYT 80134 to A.L., and IN204410 to A.Z.‐H.; O.R.‐L. is supported by a scholarship from CONACyT 240037.