Abstract
Sucrose non-fermenting 1-related protein kinases (SnRKs) comprise a major family of signaling genes in plants and are associated with metabolic regulation, nutrient utilization and stress responses. This gene family has been proposed to be involved in sucrose signaling. In the present study, we cloned three copies of the TaSnRK2.10 gene from bread wheat on chromosomes 4A, 4B and 4D. The coding sequence (CDS) is 1086 bp in length and encodes a protein of 361 amino acids that exhibits functional domains shared with SnRK2s. Based on the haplotypes of TaSnRK2.10-4A (Hap-4A-H and Hap-4A-L), a cleaved amplified polymorphic sequence (CAPS) marker designated TaSnRK2.10-4A-CAPS was developed and mapped between the markers D-1092101 and D-100014232 using a set of recombinant inbred lines (RILs). The TaSnRK2.10-4B alleles (Hap-4B-G and Hap-4B-A) were transformed into allele-specific PCR (AS-PCR) markers TaSnRK2.10-4B-AS1 and TaSnRK2.10-4B-AS2, which were located between the markers D-1281577 and S-1862758. No diversity was found for TaSnRK2.10-4D. An association analysis using a natural population consisting of 128 winter wheat varieties in multiple environments showed that the thousand grain weight (TGW) and spike length (SL) of Hap-4A-H were significantly higher than those of Hap-4A-L, but pant height (PH) was significantly lower.
Highlights
Wheat (Triticum aestivum L.) is one of the most important food crops worldwide, and obtaining higher yields is one of the primary objectives for wheat improvement
Three cDNA clones were amplified with the TaSnRK2.10-1F/R primer pair, and the corresponding gDNA sequences were amplified with the TaSnRK2.10-2F/R primer pair (S1–S3 Figs)
Each cDNA sequence of TaSnRK2.10 on 4A, 4B and 4D contained an open reading frame (ORF) of 1086 bp through ORF finder which was predicted to encode a protein of 361 amino acid residues (AARs) (Fig 1) with a molecular mass of ~40.6 kDa and a pI of ~4.80
Summary
Wheat (Triticum aestivum L.) is one of the most important food crops worldwide, and obtaining higher yields is one of the primary objectives for wheat improvement. A large number of quantitative trait loci (QTLs) have been reported to control grain yield and yield components [1,2,3,4,5,6,7]. Several yield-related genes have been cloned and transformed into functional markers (FMs), such as TaGW2 [8], TaSus2 [9], TaCwi-A1 [10], and TaGS1a [11] etc. The FMs derived from polymorphic sites in genes are important for marker-assisted selection (MAS) in breeding programs [12]. Sucrose non-fermenting 1-related protein kinases (SnRKs) form a major family of signaling proteins in plants and include three gene subfamilies, SnRK1, SnRK2 and SnRK3 [13].
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