Isolation and characterization of the most abundant rhizobacterial species associated with Vuralia turcica (Fabaceae: Papilionoideae)

DİLEK TEKDAL,CEM ÇİFTÇİ,SELİM ÇETİNER,BURÇİN ÇINGAY

doi:10.1590/0001-3765202220191460

Abstract

Vurali turcica is naturally grown in a limited area in Central Anatolia in Turkey and was categorized as a critically endangered plant in the Red Data Book of Turkish Plants. This study aimed to analyze whether the symbiotic and mutualistic relation between V. turcica rhizomes and present microflora in the habitat can be active on its distribution. Plant growth-promoting rhizobacteria (PGPRs) colonize the rhizosphere and promote plant growth and physiology. In this paper, the diversity of PGPRs of rhizomes of V. turcica was analyzed. Rhizome samples were obtained from the natural habitats of V. turcica by the workers of Nezahat Gökyiğit Botanical Garden, and bacterial isolation was conducted on the collected samples. MIS analysis, 16S rRNA, and 16S-23S rRNA ITS region sequencing were implemented, and as a result, Bacillus megaterium was found to be one of the most abundant bacterial species of the rhizomes of V. turcica based on nucleotide homology. This study is the first report on the identification of rhizobacterial species in V. turcica.

Highlights

Plant growth-promoting rhizobacteria (PGPR) affect plant growth positively through plant growth regulator production, nutrient mobilization in the soil, plant growth regulator production, inhibition, and toxic compound degradation, and plant-pathogen control (Ahemad et al 2009, Ahemad & Kibret 2014)
Secondary metabolites produced by PGPRs may have a role in facilitating nutrient uptake of plants as those metabolites convert into available forms for plant roots to absorb (Glick et al 2007)
Pre-selection of the bacteria isolated from V. turcica rhizomes

Summary

Introduction

Plant growth-promoting rhizobacteria (PGPR) affect plant growth positively through plant growth regulator production, nutrient mobilization in the soil, plant growth regulator production, inhibition, and toxic compound degradation, and plant-pathogen control (Ahemad et al 2009, Ahemad & Kibret 2014). Secondary metabolites produced by PGPRs may have a role in facilitating nutrient uptake of plants as those metabolites convert into available forms for plant roots to absorb (Glick et al 2007). Vuralia turcica (Tan et al 1983) Uysal et al 2014 belongs to the Fabaceae family and is naturally distributed in Konya province in Turkey. This species is a rare and endemic plant species. Microbial activity at the rhizomes of V. turcica has not been studied To close this gap in the literature, PGPRs living on the rhizomes of V. turcica were isolated and identified

Objectives

Methods

Results

Discussion

Conclusion