Abstract
One of the most famous spice species that lives in the tropics is black pepper (Piper nigrum L.). The study of piperine paved the way for its development as a bioactive compound in the treatment of various diseases, this is based on the ancient healing tradition of using black pepper. Our current study aims to isolate and characterize the bioactive compound piperine in Piper nigrum L. Black pepper was extracted with ethanol using the basic soxhletation and percolation methods. The results in the study showed that the extract from the Sokhlet process was yellowish moss green, and the piperine crystal color was yellow and shaped like a needle. Thin layer chromatography (TLC) results were observed using a UV-Vis spectrophotometer with different eluents and different Rf values were obtained. In addition, observations on the IR spectrum show that the piperine structure has been formed. This study provides an opportunity for a more in-depth exploration of the structure of piperine and future studies are very important to identify the potential utilization of piperine, especially as a basic material in the treatment of various diseases.
Highlights
Since the beginning of human life, spices have been an important supporting part of nutrition, and for thousands of years have been used to improve the aroma and taste of food, it is used as a preservative (Nagalingam et al, 2015)
One of the most famous spice species that lives in the tropics is Piper (Scott et al, 2007)
Black pepper was extracted with ethanol using the basic method of soxhletation and percolation
Summary
Since the beginning of human life, spices have been an important supporting part of nutrition, and for thousands of years have been used to improve the aroma and taste of food, it is used as a preservative (Nagalingam et al, 2015). Piper nigrum L. or pepper, has long been nicknamed the “king of spices” (Takooree et al, 2019), its because of the benefits in supporting human life, for example for herbal remedies, insecticides, preservatives and many other benefits (Wang et al, 2019). In 2016, Indonesia was the second largest producer of P. nigrum in the world (first Vietnam), with a total production of P. nigrum in Indonesia of 70,000 metric tons. P. nigrum continues to be cultivated and production continues to be increased (Hussain, 2017)
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