Abstract

An aldehyde oxidase gene, designated as aldehyde oxidase2 (AhAO2), was cloned from Arachis hypogaea L. The gene contained a 4,050-bp open reading frame that encoded a putative protein of 1,350 amino acids. The deduced amino acid sequence showed high identity with other plant AOs. The organ-specific expression pattern of AhAO2 has been examined, which indicates its dominant expression in leaves of peanut. The AhAO2 transcript level in leaves was greatly increased under exogenous ABA treatment for 1 h. Over-expression of AhAO2 in Arabidopsis led to an increase in ABA level and enhanced drought tolerance after drought treatment. These results indicated the possible involvement of AhAO2 in ABA synthesis and drought tolerance.

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