Abstract
α-Galactosidase treatment has been suggested as a means of removing unwanted α-galactoside sugars from legume products and from sugar beet molasses. Waste lager yeast (Saccharomyces carlsbergensis, also known as S. uvarum) was found to contain 27 units of α-galactosidase/g yeast cells (dry weight). α-Galactosidase activity was separated into 3 active peaks by DEAE cellulose chromatography. Subsequent gel filtration indicated molecular weights of the α-galactosidases in peaks A, B and C to be 118,000 daltons, 95,000 daltons and 65,000 daltons respectively. The isoelectric point of all 3 forms of the enzyme was 4.4±0.1. The enzyme of peak C had a pH optimum of 4.5. Km values ( ± standard errors) were 2.58±0.11mM p-nitrophenyl α-D-galactopyranoside (PNPG) and 16.7±1.2mM raffinose.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
