Abstract
Six temperature-sensitive (ts) mutants of Simian Virus 40 (SV40) which produce plaques at 33 but not at 40 C were isolated using either nitrosoguanidine or hydroxylamine as mutagens. One cycle of growth of the mutants at 40 C after infection with viral DNA was restricted, showing that the block to multiplication at 40 C is not due to failure in adsorption, penetration or uncoating. The mutants are more heat labile than wild-type virus, and are temperature-independent for synthesis of infectious viral DNA and for transformation. Four of the six mutants could be assigned to two complementation groups, and probably identify two genes for structural virion proteins, whose functions are not required for transformation.
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