Isolation and characterization of spinasterol from crossopterxy febrifuga stem bark

Muluh Emmanuel Khan ,Adebayo Samuel Adeiza ,T A Tor-Anyiin ,Alexander Avian

doi:10.33945/sami/pcbr.2019.2.2.5

Muluh Emmanuel Khan , Adebayo Samuel Adeiza + Show 2 more

Open Access

https://doi.org/10.33945/sami/pcbr.2019.2.2.5

Copy DOI

Abstract

In this study, stem bark of Crossopteryx febrifuga (Rubiaceae), a traditional medicinal, plant used for treatment of various diseases. The diseases was phytochemically screened by cold maceration using hexane, ethyl acetate, and methanol extracts. Results revealed the presence of bioactive compounds such as alkaloids, tannins, saponins, flavonoids, cardiac glycosides, and phytosterols. Separation and purification of the ethyl acetate extracts by column chromatography (CC), thin layer chromatography (TLC), vacuum liquid chromatography (VLC) and characterization with nuclear magnetic resonance (NMR) spectroscopic analyses, led to isolation of spinasterol, established on the basis of both 13C and 1H NMR spectral data and by comparison with literature. It was also confirmed that, spinasterol cures diabetes by reducing serum triglycerides. This is the first report on isolation of spinasterol from the stem bark of Crossopteryx febrfuga.

Highlights

Medicinal plants are a rich source of bioactive compounds which possess therapeutic properties that can be used in drug development and synthesis [1,2,3]
Due to the therapeutic nature of natural products, man has succeeded in alleviating and treating several diseases; some are yet to be properly and efficiently managed and as such they posed a great threat to humanity
Spectral Data of Phytochemicals Isolated from Crossopteryx febrifuga Stem Bark

Summary

Introduction

Medicinal plants are a rich source of bioactive compounds which possess therapeutic properties that can be used in drug development and synthesis [1,2,3]. Man has relied so much on medicinal plants for health, food, and promotion of physical and spiritual wellbeing [4,5,6,7,8]. Medicinal plants are thought to be effective and safer with less side effects and available to the common population in the underdeveloped and developing countries in the world. Due to the therapeutic nature of natural products, medicinal plants are being used as antidiabetic [9], antimicrobial [10, 11], antitrypanosomal [12], antibacterial [13], antimalarial [14], antioxidant [15], anticonvulsant [16], and antihypertensive activities [17].

Methods

Results

Conclusion