Isolation and characterization of some autochthonous microorganisms from dumpsite leachates in Ogun State, Nigeria

Abstract

Municipal solid wastes are materials generated through man's daily needs and used for life sustainability that presumably regarded as unwanted to individuals. Soil microorganisms, fungi and bacteria are known to colonize the waste, the degradation and transformation of biodegradable materials in the waste contents. The physical and chemical assessment of the leachate samples parameters measured were the hydrogen ion concentration (pH), Total Dissolved Solids (TDS), and electrical conductivity (EC) parameters, and metals such as K+, Ca2+, Mg2+, Na+, Cl-, NO3-, SO42-, and PO43-. The Aliquots from each leachate-filled bottle were drawn with a sterile pipette and used for isolating the bacterial and fungal ecology aseptically. One-milliliter of leachate samples were poured into 9 ml of distilled water and mixed thoroughly. Serial dilutions were made at 10-1 to 10-5 for the bacteria. The same dilution levels for fungi, were cultured using the pour plate method on Potato Dextrose Agar (PDA) to determine heterotrophic fungi (HF) in the leachate. The physical and chemical parameters of the leachate are Temp (℃), 27.00; pH 8. 5; TDS (mg/L), 280; BOD (mg/L), 11; EC (μS/cm), 632; K+ (mg/L, 26; Ca2+ (mg/L), 170; Mg2+ (mg/L), 32; Na+ (mg/L), 12; Cl- (mg/L), 121; NO3–(mg/L), 27; SO42- (mg/L), 58; PO43- (mg/L), 132. And the autochthonous microbial contents from the soil and leachate from the dumpsite are similar; vis: Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Penicillin sp., Rhizopus sp., Saccharomyces sp., Fusarium sp., Bacillus sp., Micrococcus sp., Corynebacterium Sp. Pseudomonas sp. and Escherichia coli.