Abstract
We report the characterization of 16 polymorphic microsatellite markers in the golden apple snail, Pomacea canaliculata, a pest registered in the list of “100 of the world’s worst invasive alien species”. The fast isolation by AFLP (Amplified Fragment Length Polymorphism) of sequences containing repeats (FIASCO) method was used to isolate microsatellite loci, and polymorphism was explored with 29 individuals collected in an invasive region from China. These primers showed a number of alleles per locus ranging from three to 13. The ranges of observed and expected heterozygosity were 0.310–0.966 and 0.523–0.898, respectively. These microsatellite markers described here will be useful for population genetic studies of P. canaliculata.
Highlights
The invasive species Pomacea canaliculata (Lamarck 1822) (Caenogastropoda, Ampullariidae), commonly known as the golden apple snail, is native to freshwater wetlands of South America [1], inInt
Only a few genetic studies have been conducted to decipher the evolutionary processes associated with the invasion of P. canaliculata [4,7]
The present study provides 16 polymorphic microsatellites isolated from P. canaliculata, which will allow investigating genetic diversity and population genetic structure of P. canaliculata in its native and invasive range, as well as tracing its invasion history
Summary
The invasive species Pomacea canaliculata (Lamarck 1822) (Caenogastropoda, Ampullariidae), commonly known as the golden apple snail, is native to freshwater wetlands of South America [1], in. Since its introduction to China for aquaculture in the 1980s, its rapid spread has caused ecological problems and great economic losses in southern provinces of China [6]. It has been listed as one of the 16 invasive species in China by the. Only a few genetic studies have been conducted to decipher the evolutionary processes associated with the invasion of P. canaliculata [4,7]. The present study provides 16 polymorphic microsatellites isolated from P. canaliculata, which will allow investigating genetic diversity and population genetic structure of P. canaliculata in its native and invasive range, as well as tracing its invasion history
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