Isolation and Characterization of Secondary Metabolite from the Stem Bark Extract of Allophylus africanus Beauv (Sapindaceae)

Abstract

Isolation of secondary metabolites from the stem bark of Allophylus africanus Beauv (Sapindaceae) was carried out. The plant has a wide distribution in West Africa, with Nigeria (Mambila Plateau, Calabar) inclusive. It has been used in communities for both its medicinal and economic importance. The stem bark of A. africanus was extracted by percolation using chloroform as solvent and concentrated by evaporation at room temperature. Single spot with an Rf value of 0.87 was obtained at a solvent mixture of hexane: chloroform at ratio 2:8 from finger column chromatography . Characterization using spectra analysis which include FT-IR, 1H-NMR, 13C-NMR, H-H COSY, H-C COSY, 135 DEPT and mass spectroscopy. Spectroscopic studies of A. Africanus stem bark extract led to the elucidation of the structure of the compound. FT-IR spectrum indicates that the compound is an aromatic derivative. Aromatic protons where seen at the 1H-NMR chemical shift values of 7.25 ppm. Olefinic protons at 5.124, 5.146 and 6.162 ppm appeared as doublets of doublets of doublets. The compound 1-((E)- 7(((1E,5E)-6-((2,3-dimethyl-4-(((E)-3,4,5,6-tetramethyldeca-7,9-dien-2-yl)oxy)pentyl)oxy)-3,4- dimethylhexa-1,5-dien-1-yl)oxy)hept-4-en-1-yl)-4-propylbenzene was isolated.