Abstract
Two compound-resistant mutants of Schizophyllum commune, InR-13 (indole resistance) and CafR-9 (caffeine resistance), were screened. The InR-13 and CafR-9 mutants were characterized by unrepressed and depressed mycelial growth on plates containing indole (350 μg/ml) and caffeine (4 mg/ml) respectively, and both of them showed flat morphology on an unselective medium. Sexually compatible mating between the mutants and wild-type compound-sensitive strains showed dominant character of specific compound resistance with fruiting in the presence of indole (60 μg/ml), and the fruit body formation of resulting crosses was advanced by the addition of either diphenylamine or benzoic acid. The partial dikaryons (semicompatible), InR13×T37 and CafR-9×T37, showed a B-common phenotype (A≠B=) with pseudoclamp and nonfruiting. This indicates that the mutations responsible for indole and caffeine resistance may affect the function of B-factor.
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