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Abstract
Abstract Ribonucleic acid isolated from cerebral cortex of mature male and female rats was fractionated by chromatography on methylated albumin Kieselguhr columns or by sucrose gradient centrifugation. Three principal fractions were obtained with analytical ultracentrifuge s20,w0 values of 4, 17, and 28. By comparison with ribonucleic acid prepared from purified rat brain ribosomes, the 17 S and 28 S fractions were shown to be predominantly ribosomal ribonucleic acid. Differences in base analyses between the three fractions were small but significant. Compositions of all fractions were markedly complementary with G ≈ C and A ≈ U.
Highlights
Ribonucleic acid isolated from cerebral cortex of mature male and female rats was fractionated by chromatography on methylated albumin Kieselguhr columns or by sucrose gradient centrifugation
Three principal fractions were obtained with analytical ultracentrifuge s~o,~values of 4, 17, and 28
By comparison with ribonucleic acid prepared from purified rat brain ribosomes,the 17 S and 28 S fractions were shown to be predominantly ribosomal ribonucleic acid
Summary
Ribonucleic acid isolated from cerebral cortex of mature male and female rats was fractionated by chromatography on methylated albumin Kieselguhr columns or by sucrose gradient centrifugation. The main portion of the paper describes RNA from male and female albino rat cerebral cortex with respect to its fractionation in a sucrose density gradient and on methylated albumin Kieselguhr columns, together with base analyses of each fraction. If RNA was to be fractionated on a methylated albumin Kieselguhr column, it was dissolved in 20 ml of 0.002 M NaCl and precipitated with 1 volume of absolute ethanol, 0.02 M magnesium acetate.
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