Abstract
ABSTRACTA bacterial strain (designated as YP1) was isolated from an aerobic granular sequence batch reactor (SBR) performing simultaneous nitrogen and phosphorus removal. Based on the morphological, biochemical characteristics, and phylogenetic analysis of 16S rRNA gene sequence, YP1 was identified as Pseudoxanthomonas sp. strain. Strain YP1 was confirmed to have the ability to conduct denitrifying phosphorus removal (DPR). The optimal conditions for YP1 were pH 8.0, phosphorus (PO43−-P) concentration of 8.0 mg/L, sodium citrate as carbon source, and nitrate nitrogen (NO3−-N) concentration of 30 mg/L. The functional genes including ppk and ppx, narG and narA, nirS and nirK were amplified for understanding the DPR pathways. The results provide more information about denitrifying polyphosphate-accumulating organisms (DPAOs) in aerobic granular sludge (AGS) and lay the foundations for full-scale DPR.
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