Abstract
Atractylodes macrocephala Koidz. (Asteraceae) is an economically important Chinese medicinal herb. In this study, 15 polymorphic microsatellite markers were developed from A. macrocephala using the compound microsatellite marker technique. Levels of polymorphism within the 15 markers were assessed using 83 individuals from two wild and two cultivated populations in China. The number of alleles per locus ranged from 2 to 20, with an average of 9.9 alleles. Observed and expected heterozygosities ranged from 0.083 to 1.000 and from 0.097 to 0.938, respectively. These markers will be valuable for germplasm classification and identification, as well as for assessing the genetic diversity and spatial genetic structure among wild and cultivated populations of A. macrocephala.
Highlights
Atractylodes macrocephala Koidz. (Asteraceae) is a perennial herb that is endemic to several provinces in China
Eighty-three individuals of A. macrocephala (Supplementary Table S1) from two cultivated populations (Panan County, Zhejiang Province and Pingjiang County, Hunan Province) and two wild populations (Jiulong Mountain, Hunan Province and Micang Mountain, Shaanxi Province) in China were employed to test the efficiency of the locus-specific primer pairs and to characterize these microsatellite markers
Among the 20 primer pairs, 15 were polymorphic and 5 were monomorphic within all four populations of A. macrocephala, though these monomorphic markers could be potentially useful for other populations not tested [12] or other related species
Summary
Atractylodes macrocephala Koidz. (Asteraceae) is a perennial herb that is endemic to several provinces in China. Rhizoma (common name: “Baizhu” in Chinese and “Byakujutsu” in Japenese), is widely used in traditional herbal remedies in Asia [1,2] It was reported as a nutrient for energy and for the treatment of dyspepsia and anorexia [3]. Eastern Zhejiang Province is well known for its high quality Atractylodes Macrocephala Rhizoma, while Bozhou City in Anhui Province is the largest producer. A. macrocephala is a perennial herb, which can reproduce sexually through cross-pollination [9]. It is cultivated as a biennial crop by seed in China and is generally harvested after the fruits ripen. We report the isolation and characterization of the first set of polymorphic microsatellite markers for this species and expect these to provide a useful tool for population genetic studies
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