Abstract
Cupressus funebris Endl. (Cupressaceae) is an endemic conifer species in central and eastern China. In order to investigate the genetic diversity within and between populations and design the effective conservation strategies, we aimed to develop microsatellite markers for this species in the present study. We isolated and characterized 12 novel polymorphic microsatellite loci for this species through the combined biotin capture method. Polymorphism of each locus was further assessed through 62 individuals from three geographically distant populations. The number of alleles per locus ranged from 5 to 12. The observed and expected heterozygosity per locus ranged from 0.115 to 0.396, and 0.204 to 0.542, respectively. One locus (Cf07) showed significant deviation from Hardy–Weinberg equilibrium and no Linkage disequilibrium was detected after Bonferroni corrections. These microsatellite markers will be useful for population genetics studies of this conifer species.
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