Abstract
Here we developed and optimized 15 polymorphic microsatellites isolated from Cherax quadricarinatus enriched for CA repeats. We tested variability of these microsatellites in 60 unrelated individuals cultured in China. All microsatellite loci were polymorphic. Number of alleles per locus ranged from 2-7 while observed and expected heterozygosities ranged from 0.2549 to 0.8615 and from 0.3405 to 0.8174, respectively. Fourteen of the 15 microsatellites conformed to Hardy–Weinberg Equilibrium. These microsatellite loci developed here provide an important resource for studying genetic diversity and population structure in redclaw crayfi sh and potentially in other related species.
Highlights
The redclaw crayfish Cherax quadricarinatus accounts for the entire commercial production of freshwater crayfish in Australia [1]
Isolation of more microsatellite loci from C. quadricarinatus will be important for elucidating population structure and for monitoring relative levels of genetic diversity in sampled populations
We screened 72 recombinant clones with M13 and (CA)10 primers, 45 selected clones with repeat elements were sequenced with M13 universal primers (Invitrogen)
Summary
The redclaw crayfish Cherax quadricarinatus accounts for the entire commercial production of freshwater crayfish in Australia [1]. Genetic markers can characterize relative levels of population variation and differentiation in both wild and cultured stocks [2]. Our work on SSR markers isolation and studies of genetic diversity and population structure will provide important information for promoting development of aquaculture of C. quadricarinatus in China.
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