Isolation and Characterization of Pectic Polysaccharide Fraction fromIn VitroSuspension Culture ofFumaria officinalisL.

Manol Ognyanov,Nadezhda Petkova,Ivan Ivanov,Yordan Georgiev,Ivelina Vasileva,Maria Kratchanova

doi:10.1155/2018/5705036

Abstract

In the current study, an acidic polysaccharide from thein vitrosuspension culture ofFumaria officinalisL. was obtained by extraction with 0.8% (w/v) aqueous ammonium oxalate. The polysaccharide fraction mainly consisted of galacturonic acid (41.0%), followed by galactose (7.3%) and arabinose (5.6%). This suggests the presence of arabinogalactan side chains in the rhamnogalacturonan-I segment of the studied pectin, which was mainly built up by homogalacturonan segments. The pectin was evaluated as low-methyl-esterified (45.0%) with degree of acetylation 3.4%. The polymer fraction was consisted of different molecular weight populations in the range of 6–600 kDa. The high amount of 4-L-hydroxyproline (11.7% of total protein) and the specific positive reaction to Yariv’s phenylglycoside reagent indicated the presence of an arabinogalactan protein in the cell walls. The functional properties of the polysaccharide fraction were evaluated, as it possessed better water-holding capacity than oil-holding capacity. The studied pectin demonstrated significant foaming ability and promising emulsifying properties in a concentration 1%. Therefore, the isolated polysaccharide fraction could be successfully used as emulsifier and foaming agent in food products and pharmaceutical supplements.

Highlights

Fumaria officinalis L., known as common fumitory, drug fumitory, or earth smoke, is a plant of Fumaria genus that belongs to the Fumariaceae family
We showed the presence of acidic fraction in the cell walls of suspension culture of Fumaria officinalis L
The fraction predominantly consisted of linear HG segments and characterized as typical low-esterified pectic polysaccharide

Summary

Introduction

Fumaria officinalis L., known as common fumitory, drug fumitory, or earth smoke, is a plant of Fumaria genus that belongs to the Fumariaceae family. It is widely distributed in particular regions of Europe (e.g., the Balkan Peninsula, Mediterranean region). The presence of isoquinoline alkaloids, the most important of which is protopine, is mainly associated with the biological activity of Fumaria spp. The total polyphenol content and antioxidant activity of five Bulgarian Fumaria spp., including F. officinalis L., were evaluated [4]. Detailed descriptions of pharmacological activities of polysaccharides isolated from herbs were subject of many reports [5, 6].

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Conclusion