Abstract
Using Vero cells, we isolated a virus (NII561-2000) from a cerebrospinal fluid specimen of a 1-year-old girl with Reye syndrome. The determined amino acid sequence of the virus indicated that the isolate was a human parechovirus (HPeV), a member of Picornaviridae. Neutralization test showed that the NII561-2000 virus had distinct antigenicity to HPeV-1, HPeV-2, and HPeV-3, and that the sequence was distinct from these types as well as from HPeV-4 and HPeV-5. Thus, we propose the virus (NII561-2000) as the prototype of HPeV-6. We isolated 10 NII561-2000-related viruses, 14 HPeV-1, 16 HPeV-3, and 1 HPeV-4 of 41 HPeVs from various clinical samples collected in Niigata, Japan. Clinical symptoms of the persons infected with the NII561-2000-related viruses were infectious gastroenteritis, rash, upper respiratory tract infection, and paralysis, in addition to Reye syndrome in the 1-year-old girl.
Highlights
Using Vero cells, we isolated a virus (NII561-2000) from a cerebrospinal fluid specimen of a 1-year-old girl with Reye syndrome
We report a 1-year-old girl who died with Reye syndrome, which is characterized as an acute, noninflammatory encephalopathy with hepatic dysfunction and fatty infiltration of the viscera; the syndrome is frequently associated with an antecedent viral infection, such as influenza or varicella [10,11,12]
Vero cells inoculated with the specimen exhibited a cytopathic effect (CPE)
Summary
Using Vero cells, we isolated a virus (NII561-2000) from a cerebrospinal fluid specimen of a 1-year-old girl with Reye syndrome. The determined amino acid sequence of the virus indicated that the isolate was a human parechovirus (HPeV), a member of Picornaviridae. ≈20% of healthy children in Finland have antibodies against HPeV-1, and the percentage is as high as 97% in adults [8] These viruses are frequently isolated from patients with various human diseases, such as gastroenteritis, encephalitis, flaccid paralysis, and respiratory infections, and they are thought to be associated with these diseases [2,3,5,8,9]. We propose that NII561-2000 is the prototype of HPeV-6
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