Abstract
Abstract Several nondefective, tryptophan-transducing derivatives of bacteriophage φ80 (φ80 pt) have been isolated which carry various segments of the tryptophan operon. Tryp+ transductants arising from infection by φ80 pt phages are distinguished from those resulting from defective transducing phages by having rough edges and lytic halos. The most frequently encountered type of φ80 pt is that carrying all the tryptophan genes except that specifying anthranilate synthetase. This suggests that in most events leading to transducing phage formation, a specific region in the tryptophan operon interacts with a homologous prophage region. Mutations in the tryptophan operon including both the localized ones and those of the T1r trypdel type can be rapidly mapped employing transduction frequency tests with the various φ80 pt phages. No correlation was found between buoyant densities of the φ80 pt phages and the amount of tryptophan genes incorporated into their genomes. This suggests that during formation of transducing phage unequal exchange of bacterial and phage genetic material occurs.
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