Abstract
A cDNA sequence coding for a human interleukin 4 (IL-4) has been isolated from a concanavalin A-activated human T cell cDNA library based on homology with a mouse interleukin 4 cDNA that expresses BSF-1 activity and T cell- and mast cell-stimulating activities (Lee, F., et al., Proc. Natl. Acad. Sci. USA, 1986, 83:2061; Yokota, T., et al., ibid. 1986, 83:5894). Supernatants of C0S7 monkey cells transfected with the human cDNA clone stimulate proliferation of human T cell clones and anti-IgM-activated human B cells and induce Fc∈ receptors and MHC Class II molecules on human B cells (all properties of mouse BSF-1 on mouse cells). These results indicate that the human IL-4 cDNA clone encodes a protein structurally and functionally homologous to mouse IL-4. Both mouse and human IL-4 genes are organized in a similar manner and are composed of three introns and four exons. Highly conserved sequences exist in both the mouse and human IL-4 genes. In addition, using expression cloning, we have isolated from activated T cells a mouse cDNA clone which stimulates B cells to produce IgA and has eosinophil-CSF activity on human cord blood cells, and a homologous human cDNA clone from a human T cell cDNA library. Sequence analysis reveal that these clones are identical to TRF1 or BCGFII cDNA clones isolated previously (Kinashi, T., et al., Nature 1986 324: 7, Azuma et al., Nuc. Acid. Res. 1986 14: 9149).
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