Abstract
A monoclonal antibody 62-1A of isotype IgM, directed against bovine αs2-casein, was isolated and characterized. Monoclonal antibody 62-1A recognized bovine αs2-11P- and αs2-9P-caseins by indirect solid phase radioimmunoassay and Western blot analysis. Crossreactivity toward native genetic variants (A, B, and C) of αs1-casein was similar but lower (˜60 to 70%) than that for αs2-11P-casein). Little or no crossreactivity was observed for other bovine milk or serum proteins. Antibody affinity for αs2-11P-casein was 1.3 × 109/M. As little as 25ng/ml (.5 ng/well) of αs2-11P-casein was detected by solid phase radioimmunoassay.
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